Effects of Dioscin on DNA Methyltransferase 1 Protein Expression in Human Breast Cancer Cell Lines

Means, David Andrew (2017) Effects of Dioscin on DNA Methyltransferase 1 Protein Expression in Human Breast Cancer Cell Lines. Undergraduate thesis, under the direction of Asok Dasmahapatra from Pharmacology, The University of Mississippi.

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In previous experiments, the steroidal saponin, dioscin, which was extracted from the roots of the wild yam (Dioscorea villosa), was observed to have potent anti-proliferative effects on the human breast cancer cell lines MCF-7 and MDA-MB-231. Dioscin was also able to activate the transcription of tumor suppressor genes GATA3 and CDH1 in these cell lines. We hypothesize that the anti-proliferative effect of dioscin on human breast cancer cells could be mediated through the epigenome. Consequently, in order to explore this hypothesis, we have analyzed the expression of DNA methyltransferase 1 (DNMT1) protein in these cells. MCF-7 and MDA-MB-231 cells were treated with differing concentrations of dioscin, as well as a 1μM concentration of the known DNMT1 inhibitor, 5-azacytidine. Protein lysate was collected from these cells and was then subjected to Western blot analysis. Both dioscin and 5-azacytidine decreased protein expression of the DNMT isoform with a molecular weight of 145 kDa. It may be concluded that dioscin has potential DNMT1 inhibiting ability, but whether or not this ability directly contributes to dioscin’s anti-proliferative effects and the activation of the tumor suppressor genes GATA3 and CDH1 remains to be seen.

Item Type: Thesis (Undergraduate)
Creators: Means, David Andrew
Student's Degree Program(s): B.S. Biology
Thesis Advisor: Asok Dasmahapatra
Thesis Advisor's Department: Pharmacology
Institution: The University of Mississippi
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Depositing User: Mr. David Means
Date Deposited: 11 May 2017 17:01
Last Modified: 11 May 2017 17:01
URI: http://thesis.honors.olemiss.edu/id/eprint/826

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