Effect of 5-hydroxymethylcytosine modifications on stability of VEGF i-Motifs

Summerford, Bethany (2016) Effect of 5-hydroxymethylcytosine modifications on stability of VEGF i-Motifs. Undergraduate thesis, under the direction of Randy Wadkins from Chemistry & Biochemistry, University of Mississippi.

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DNA i-motifs are a secondary structure of DNA formed at low pH by internal folding of strands rich in cytosine. This study focuses on trying to determine if the DNA from the vascular endothelial growth factor (VEGF) promoter, which can form an i-motif, can be stabilized with the epigenetic modification to 5- hydroxymethylcytosine in four separate locations on the DNA strand, and to what extent the degree of stabilization depends on the location of the modification. Stability of the i-motif was determined by measuring pKa and melting point (Tm) of each VEGF modification using circular dichroism spectroscopy. The modified samples showed an insignificant change in pKa values but a significant increase in Tm when compared to the wild type, indicating stabilization by epigenetic modification. The ability to stabilize i-motifs has potential for using i-motif DNA in new drug delivery methods.

Item Type: Thesis (Undergraduate)
Creators: Summerford, Bethany
Student's Degree Program(s): B.A. in Biochemistry
Thesis Advisor: Randy Wadkins
Thesis Advisor's Department: Chemistry & Biochemistry
Institution: University of Mississippi
Subjects: Q Science > QD Chemistry
Depositing User: Bethany Summerford
Date Deposited: 12 May 2016 18:26
Last Modified: 12 May 2016 18:26
URI: http://thesis.honors.olemiss.edu/id/eprint/511

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