Neurotoxic Effects of HIV-1 gp120 and Interaction with Allopregnanolone

Thornton, Kimberly A. (2019) Neurotoxic Effects of HIV-1 gp120 and Interaction with Allopregnanolone. Undergraduate thesis, under the direction of Jason Paris from BioMolecular Sciences, The University of Mississippi.


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With increasing incidence of HIV and its associated neurocognitive disorders, there has emerged a need for an antiviral with improved delivery to the CNS. In searching for drug candidates that meet these criteria, some have noted that certain steroid hormones like estrogen and progesterone that naturally accumulate in the CNS show neuroprotective effects in cells exposed to HIV proteins. This investigation focused on testing the potential protective effects of the progesterone metabolite, allopregnanolone (AlloP), against HIV protein gp120-mediated neurotoxicity. Experiments were conducted using SH-SY5Y neuroblastoma cells that were exposed to varying concentrations of AlloP (0-100 nM) in conjunction with one of three gp120 conditions (vehicle, X4 gp120 at 500pM, R5 gp120 at 500 pM). Once treated, plates were imaged at 24-hour intervals for 48 hours and assessed for proportion of cell death. Analysis of the results found that AlloP had no significant influence over cell death and that gp120 only induced a significant amount of cell death once deglycosylated. Further investigation will be needed to assess the influence of AlloP and natural steroid products on the direct and indirect glial-mediated neurotoxic mechanisms of HIV-1 gp120.

Item Type: Thesis (Undergraduate)
Creators: Thornton, Kimberly A.
Student's Degree Program(s): B.S. in Pharmaceutical Sciences
Thesis Advisor: Jason Paris
Thesis Advisor's Department: BioMolecular Sciences
Institution: The University of Mississippi
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
R Medicine > RM Therapeutics. Pharmacology
Depositing User: Kimberly Ann Thornton
Date Deposited: 10 May 2019 17:47
Last Modified: 10 May 2019 17:47

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