Cell-free telomere DNA as a biomarker for treatment response and tumor burden in Glioblastoma

North, John P. (2018) Cell-free telomere DNA as a biomarker for treatment response and tumor burden in Glioblastoma. Undergraduate thesis, under the direction of Nathan Hammer from Chemistry and Biochemistry, University of Mississippi.

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Telomeres are repeated nucleotide sequences that cap the ends of each chromosome in eukaryotes. Telomeres are among the earliest genomic sequences to be degraded during apoptosis, potentially providing a biomarker of cell death. To study this, we quantified telomeres in serum from Glioblastoma and non-cancer patients. Cell-free DNA was isolated by centrifugation to remove intact cells, and purified using a QIAamp DNA Blood Midi Kit (Qiagen). Cell-free telomeric (cf-tel) and cell-free actin (cf-actin) DNA were analyzed with quantitative PCR. Total cell-free DNA was measured with PicoGreen assays. We hypothesize that patients with Glioblastoma tumors have higher cf-tel DNA levels than those without tumors. Our results indicate that cf-tel DNA was present at nearly double the amount in Glioblastoma patients when compared to non-cancer control patients with a significant difference (p=0.0151), while cf-actin DNA and total cell-free DNA amounts for Glioblastoma patients and non-cancer control patients were nearly identical. We conclude that cell-free telomeric DNA can be detected and measured in serum from normal patients and patients with a history of Glioblastoma, the increased presence of cell-free telomeric DNA is directly correlated with Glioblastoma disease conditions, and cell-free telomeric DNA may be a useful clinical biomarker for treatment response and for measurement of tumor burden.

Item Type: Thesis (Undergraduate)
Creators: North, John P.
Student's Degree Program(s): B.A. in Biochemistry
Thesis Advisor: Nathan Hammer
Thesis Advisor's Department: Chemistry and Biochemistry
Institution: University of Mississippi
Subjects: Q Science > QD Chemistry
Depositing User: Mr. John North
Date Deposited: 11 May 2018 18:54
Last Modified: 11 May 2018 18:54
URI: http://thesis.honors.olemiss.edu/id/eprint/1163

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