Exploration of Compound Viability as an Organ-Selective Anti-Metastatic Therapeutic Treatment for Triple Negative Breast Cancer

Noblin, Ashley E. (2016) Exploration of Compound Viability as an Organ-Selective Anti-Metastatic Therapeutic Treatment for Triple Negative Breast Cancer. Undergraduate thesis, under the direction of Dale Nagle from BioMolecular Sciences, University of Mississippi, School of Pharmacy.

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Abstract

Phenformin and metformin are compounds that are currently used to treat Type II Diabetes and have been shown to suppress breast cancer metastasis. These compounds were tested, after extensive research and screening of current approved oncology drugs, in various experiments with the cell lines MDA-MB-231, LM, and BoM, with MDA-MB-231 being the parental cell line, LM being the lung metastatic line, and BoM being the bone metastatic line. The cells were treated with compound addition at various concentrations and subject to a two-day concentration response viability study in order to determine any inhibitory effects, followed by a five-day assay in order to determine the effect of extended exposure duration. Phenformin was found to act inconsistently at physiologically relevant concentrations. Metformin, however, showed significant increases in inhibitory effects when the cells were exposed to the compound for a longer duration. Clonogenic assays were performed to confirm the inhibitory effects of the compounds. Further pharmacology research needs to be completed, but both compounds show potential to serve as an antimetastatic therapeutic treatment option and thus improve the prognosis of breast cancer patients.

Item Type: Thesis (Undergraduate)
Creators: Noblin, Ashley E.
Student's Degree Program(s): Biology
Thesis Advisor: Dale Nagle
Thesis Advisor's Department: BioMolecular Sciences
Institution: University of Mississippi, School of Pharmacy
Subjects: >
Depositing User: Ashley Noblin
Date Deposited: 16 Jun 2016 15:55
Last Modified: 16 Jun 2016 15:55
URI: http://thesis.honors.olemiss.edu/id/eprint/703

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