Not Got Milk? The Effect on the Gut Microbiome of Removing Dairy from the Diet

Stovall, Patrick Tyler (2015) Not Got Milk? The Effect on the Gut Microbiome of Removing Dairy from the Diet. Undergraduate thesis, under the direction of Colin Jackson from Biology, The University of Mississippi.

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Abstract

ABSTRACT Patrick Tyler Stovall: Not Got Milk? The Effect on the Gut Microbiome of Removing Dairy from the Diet (Under the direction of Colin R. Jackson, Ph.D.) The human gut contains a highly diverse set of bacteria that perform a wide range of duties that include much more than just nutrient acquisition. However, the composition of this community is subject to change, with diet, age and lifestyle playing roles in the development and maintenance of the gut microbiota. This study compared the bacterial composition of the human gut when consuming a normal diet versus a dairy-free diet. Samples were taken from a single subject during three periods: 1) control (normal) diet, 2) dairy-free diet, and 3) a return to normal diet. Gut bacterial communities were identified and compared using 16S rRNA gene sequencing. Relative to the total number of sequences within a sample, abundances were calculated for dominant bacterial groups starting at the phylum level and progressing to the smallest identifiable taxonomic group. Fluctuations were seen at taxonomic levels from class down to species. Non-metric multidimensional scaling ordination revealed that the samples from each dietary period were distinguishable from the other periods. Six significant operational taxonomic units (OTUs), from three phyla, were significantly related to dietary sample distributions. These OTUs consisted of two members of the Bacteroidetes (both genus Bacteroides), three from Firmicutes (genus Megasphaera, genus Acidamniococcus, and Butyricicoccus pullicaecorum), and one from Actinobacteria (Collinsella aerofaciens). This study shows that alterations to a diet can cause changes of the relative abundances of bacteria in the human gut at multiple taxonomic levels, but that at the level of the entire community these shifts in gut microbiota can be reversed.

Item Type: Thesis (Undergraduate)
Creators: Stovall, Patrick Tyler
Student's Degree Program(s): Biology
Thesis Advisor: Colin Jackson
Thesis Advisor's Department: Biology
Institution: The University of Mississippi
Subjects: Q Science > QR Microbiology
Depositing User: Patrick Stovall
Date Deposited: 07 May 2015 16:11
Last Modified: 07 May 2015 16:11
URI: http://thesis.honors.olemiss.edu/id/eprint/372

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