The Effects of Hg2+ on Secondary Structures Formed by T-Rich DNA

Boynton, Zachary (2015) The Effects of Hg2+ on Secondary Structures Formed by T-Rich DNA. Undergraduate thesis, under the direction of Randy Wadkins from Chemistry and Biochemistry, University of Mississippi.

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Abstract

Single stranded DNA that forms secondary structures has been a popular area of research in recent years. Much research has also been done in the field of heavy metals binding to DNA. Recently, it has been discovered that mercury in the presence of thymines will form T-HgII-T bonds. It is our hypothesis that single stranded DNA rich in thymine residues will form into a structure similar to i-motifs, formed from C-rich single strands, in the presence of mercury. Based on spectral changes during a mercuric titration, it is evident that mercury induces a structural change in T-rich DNA. We then tested the thermal stability of this structure via heat-induced denaturation. Job Plots were constructed to determine the number of Hg2+ binding sites on the DNA and size-exclusion chromatography experiments were conducted to determine if T-I-motif’s are formed inter- or intra-molecularly.

Item Type: Thesis (Undergraduate)
Creators: Boynton, Zachary
Student's Degree Program(s): B.A. Biochemistry
Thesis Advisor: Randy Wadkins
Thesis Advisor's Department: Chemistry and Biochemistry
Institution: University of Mississippi
Subjects: Q Science > QD Chemistry
Depositing User: Zachary Boynton
Date Deposited: 07 May 2015 16:01
Last Modified: 07 May 2015 16:01
URI: http://thesis.honors.olemiss.edu/id/eprint/366

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