Identification of a Phytotoxic Fungus and an Investigation into the Isolation of its Phytotoxic Constituents

Briscoe, William E. (2014) Identification of a Phytotoxic Fungus and an Investigation into the Isolation of its Phytotoxic Constituents. Undergraduate thesis, under the direction of Randy Wadkins from Department of Chemistry and Biochemistry , University of Mississippi.

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Abstract

A fungus was isolated from Hedera helix (English ivy) leaves experiencing necrosis and identified as Diaporthe eres by molecular techniques. The fungus was grown in potato dextrose broth medium and the culture medium was extracted with ethyl acetate. Bioassay guided fractionation of ethyl acetate extract of culture filtrate of this fungus led to the isolation of two phytotoxic compounds that are active against the seed germination of the monocotyledon Agrostis stolonifera at 1 mg mL-1. Structures of these active compounds were determined by spectroscopic methods and were determined 8-hydroxy-3.7-dimethylisochroman-1-one and 2-(4-hydroxyphenyl)-ethanol. This is the first finding presented on the phytotoxic effects of as 8-hydroxy-3.7-dimethylisochroman-1-one.

Item Type: Thesis (Undergraduate)
Creators: Briscoe, William E.
Student's Degree Program(s): B.A. in Biochemistry
Thesis Advisor: Randy Wadkins
Thesis Advisor's Department: Department of Chemistry and Biochemistry
Institution: University of Mississippi
Subjects: >
Depositing User: Mr. William Briscoe
Date Deposited: 27 Jan 2015 19:45
Last Modified: 27 Jan 2015 19:45
URI: http://thesis.honors.olemiss.edu/id/eprint/251

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