Microbial Communities Associated with Dragonfly Nymphs Raised in Varying Concentrations of Amoxicillin

Basha, Michelle (2019) Microbial Communities Associated with Dragonfly Nymphs Raised in Varying Concentrations of Amoxicillin. Undergraduate thesis, under the direction of Colin Jackson from Biological Sciences, The University of Mississippi.

[img]
Preview
Text
Basha_thesis.pdf

Download (273kB) | Preview

Abstract

The bacteria on and within an organism make up that organism’s microbiome. Given interest in the use of antibiotics in agriculture and the effect of microbes on human health, more studies are needed on the microbial community composition of different organisms and how it responds to antibiotic use. This study investigated changes in the amount of antibiotic resistant bacteria present in dragonfly nymphs exposed to differing concentrations of amoxicillin. Next generation sequencing of the 16S rRNA gene was used to identify cultures of these antibiotic resistant bacteria. Increasing the concentration of antibiotics the dragonfly nymphs were exposed to resulted in greater numbers of antibiotic resistant bacteria. From both amoxicillin + TSA and TSA-only plates, Proteobacteria was the most abundant phyla detected. Bacteroidetes was the major phyla detected in nymphs raised in 0% amoxicillin and plated on amoxicillin + TSA plates. There was a high relative proportion of members of the phylum Firmicutes in all samples plated on TSA plates. In the nymphs raised in 0% amoxicillin plated on TSA plates, members of Firmicutes made up the majority of their microbiome. This study demonstrates that the bacterial communities associated with dragonfly nymphs are affected by changes in the environment, and that exposure to antibiotic pollution likely increases the number of antibiotic resistant bacteria within aquatic insects.

Item Type: Thesis (Undergraduate)
Creators: Basha, Michelle
Student's Degree Program(s): B.S. in Biology
Thesis Advisor: Colin Jackson
Thesis Advisor's Department: Biological Sciences
Institution: The University of Mississippi
Subjects: Q Science > QR Microbiology
Depositing User: Michelle Basha
Date Deposited: 10 May 2019 03:44
Last Modified: 10 May 2019 03:44
URI: http://thesis.honors.olemiss.edu/id/eprint/1368

Actions (login required)

View Item View Item