Characterizing the Effects of stm and ath1 Mutations on Floral Organ Development in Arabidopsis thaliana

Leary, James Luke (2018) Characterizing the Effects of stm and ath1 Mutations on Floral Organ Development in Arabidopsis thaliana. Undergraduate thesis, under the direction of Sarah Liljegren from Biology, University of Mississippi.

[img]
Preview
Text
Luke Leary Thesis Final Draft for HOCO.pdf

Download (791kB) | Preview

Abstract

JAMES LUKE LEARY: Characterizing the Effects of stm and ath1 Mutations on Floral Organ Development in Arabidopsis thaliana(Under the direction of Dr. Sarah Liljegren) The purpose of this project was to explore the function of SHOOTMERISTEMLESS (STM) and ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1), two transcription factors in Arabidopsis thaliana that play a role in the molecular pathways that establish organ boundaries in flowers. Prior research has shownthat mutations in the STM and ATH1 genes cause defects in the boundaries formed between floral organs and the stem of the plant. My study was designed to investigate whether ATH1 and STM also control the boundaries found between adjacent floral organs. I predicted that stm ath1-3 double mutant flowers would display a significant number of stamen-stamen and sepal-sepal fusion events compared to wild-type flowers, which would indicate that the boundaries between these organs had been compromised. Stem cells found in flower meristems are required for a full set of floral organs to develop. Since STM are ATH1 are known to play roles in maintaining the population of stem cells in both shoot and flower meristems, stm ath1-3 double mutant flowers were also expected to produce fewer organs compared to wild-type flowers. I found that 15 percent of the stamens in stm and ath1-3 single mutant flowers, and 51 percent of the stamens in stm ath1-3 double mutant flowers were fused to another stamen. I also found that 95 percent of the sepals in the double mutant flowers were fused to a neighboring sepal. The stm ath1-3 mutant flowers produced three fewer petals and three fewer stamens on average. These results indicate that STM and ATH1 redundantly regulate boundaries formed between stamens and petals. They also demonstrate that STM and ATH1 regulate the size of the flower meristem and that their functions are required for that meristem to correctly produce all of its organs.

Item Type: Thesis (Undergraduate)
Creators: Leary, James Luke
Student's Degree Program(s): Bachelor of Arts: Biology
Thesis Advisor: Sarah Liljegren
Thesis Advisor's Department: Biology
Institution: University of Mississippi
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: MR. James Luke Leary
Date Deposited: 14 May 2018 13:20
Last Modified: 14 May 2018 13:20
URI: http://thesis.honors.olemiss.edu/id/eprint/1178

Actions (login required)

View Item View Item